Journal:

Article Title: Characterization of Monoclonal Antibodies Raised against the Latent Nuclear Antigen of Human Herpesvirus 8

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Figure Lengend Snippet: LN53 reactivity to LNA in nodular KS. Immunocytochemistry was performed on a paraffin-embedded tumor from classical KS. Permeabilization was performed by microwave. Negative control biopsies were from angiosarcomas and hemangiomas (not shown). After incubation with normal rabbit serum (DAKO), MAbs were applied for 1 h at 22°C followed by two washes in PBS–0.1% (vol/vol) Tween. The secondary biotin-conjugated antibody (rabbit anti-rat; DAKO) was applied for 30 min followed by washing. Antibody reactions were visualized with streptavidine-alkaline phosphatase (Vector Laboratories) and a substrate red chromogen (Vector). Adjacent sections were stained by standard methods. (a) Hematoxylin and eosin staining showing KS nodule (left) and surrounding dermis (right). (b) Adjacent sections stained with LN53 showing clear demarcation between LNA expression in the KS lesion and not in the surrounding dermis (magnification, ×40). Almost all spindle-shaped cells are positive for LNA (×60) (c), with a stippling pattern reminiscent of the staining of PEL cells (×160) (d).

Article Snippet: Antibody reactions were visualized with streptavidine-alkaline phosphatase (Vector Laboratories) and a substrate red chromogen (Vector).

Techniques: Immunocytochemistry, Negative Control, Incubation, Plasmid Preparation, Staining, Expressing